奈米工程與微系統研究所　書報討論

Seminar of Inst. NanoEngineering and MicroSystems

陳之碩 助理教授

Assistant Prof. Chi-Shuo Chen

Department of Biomedical Engineering and Environmental Science, National Tsing Hau University

E-mail : chen.cs@mx.nthu.edu.tw

Mechanotransduction at cellular and molecular levels

Abstract

Mechanotransduction plays fundamental roles in tissue integrity and cancer metastasis. Membrane adherence junctions (AJs) proteins and cytoskeletons can assemble into complex molecular framework, however, the organization and dynamics of those key molecular structures remain underexplored.  Using Single Molecule Localization Microscopy, we showed cadherin clusters with crystal-like density. We investigated how actins regulate AJs dynamic, and results of Photoactive fluorescent microscopy showed altered stability of actin structures can contribute to the AJs formations. Furthermore, a traction force microscopy (TFM) was developed to quantify the mechanical interactions at cellular level. In summary, we demonstrated the actin dynamic associates with the formation of AJs, which can contribute to the observed high cell proliferation and cell invasion. These findings are expected to contribute to our current understanding about actin cytoskeleton  through mechanotransduction perspective.

Profile

Dr. Chi-Shuo Chen is an Assistant Professor of Department of Biomedical Engineering and Environmental Sciences at National Tsing Hua University. He received his B.S and M.S in Atomic Science from National Tsing Hua University, and Ph.D. in Biological Engineering from University of California, Merced. He worked as a Postdoctoral Researcher in Dermatology at Feinberg School of Medicine, Northwestern University. His current research focus is to study how mechanical signals propagate between cells, and the related mechanotransduction responses within cellular structures. By integrating with various engineering platforms, such as microfluidic, optical tweezers system, and other microscopy technologies, the works developed in his laboratory aims to further understand the impacts of physical microenvironments on cell physiology. His works were published at journals among biomedical field, including PNAS, Journal of Cell Biology, Scientific Report, and Lab on a chip, etc.

15:30-17:20 on Oct. 15 (Mon), 2018 in Room 108 of Eng. Building I

Host：Prof. Da-Jeng Yao  djyao@mx.nthu.edu.tw Ext: 42850

Contact：黃微稜 hwleng@mx.nthu.edu.tw  Ext: 33737

TA：李志恩 c27180339@gmail.com