奈米工程與微系統研究所　書報討論

Seminar of Inst. NanoEngineering and MicroSystems

劉昭沅 博士/Chao-Yuan Liu

國立台灣大學 National Taiwan University

New Applications of Advanced Direct Coating and Hybrid Machining for Immuno-Detection and Biomedical Devices

Conventional immuno-detection consumes large amount of resources and have a major defect in signal sensitivity and time-intensive procedures. In Taiwan, In Vitro Diagnostic sector contributes to about 3 billion USD annually; however, the output value of domestic testing reagent is still less than 10% of the total market [1]. The overuse of antibody is one major portion of such market value. As an alternative, several methods using different design concepts have been introduced by industries (e.g., EMD Millipore) [2] to increase the efficiency and sensitivity of Western blotting, the typical immune-probing. Our work explored the feasibility of using direct coating technology as a convincing strategy to shorten the operation time and reagent consumption by utilizing a novel compact silicon coater. As a result, the thin film direct coating with rapid suction (TDCS) technology could provide a 6-log2 dynamic detection range, much shorter operation time of about 5 min (1/36 of the total processing time required in conventional WB) or even less (through the system optimization). Furthermore, compared with conventional WB, the consumption of primary antibody can be substantially reduced by a factor of 1/20,000 [3-4]. Apart from coating, we can also incorporate the knowledges of microfluidics achieved by hybrid machining with high biocompatibility polyetheretherketone (PEEK) material to further miniaturize the system. In our knowledge, customized PEEK structure is still lacking on the market because of its high melting point (343°C) and high viscosity (380,000 cp). Recently, a newly startup medical additive manufacturing (AM) center co-funded by Show Chuan Memorial Hospital (SCMH) and National Taiwan University (NTU) showed the feasibility on fabricating customized PEEK structure. This work was intended to make not only the microfluidic chips for medical detection, but also an initial study on how effective that artificial intervertebral PEEK cage would perform inside the human body [5]. By utilizing the self-developed multi-axes motion control with 6 degree of freedom, the experimental results showed that the printing speed was increased up to 265 mm/sec from the common limit (e.g., ~60 mm/sec for nozzle in 400 μm diameter) of filament feeding with flow rate variation within 3%. 3D structures and artificial intervertebral cage with designed porosity have been successfully manufactured. It is highly expected that the patients can be benefited through the customized filling(s) with a much shorter recovery time after the operation in the coming future.

15:30-17:20 on MAY. 01 (Mon), 2017 in Room 108 of Eng. Building I

主持人/Host：傅建中 教授/Prof. Chien-Chung Fu

聯絡人/Contact：吳崇瑄 先生/Mr. Chong-Syuan Wu 0911971628