奈米工程與微系統研究所　書報討論

Seminar of Inst. NanoEngineering and MicroSystems

游佳欣 副教授

國立台灣大學 化工系

Associate Prof. Jiashing Yu,

Department of Chemical Engineering, National Taiwan University.

E-mail: jiayu@ntu.edu.tw

Poly(3,4-ethylenedioxythiophene)-based Bioelectrodes with Designed Chemical and Topographical Cues to Manipulate the Behavior of Neural Cells

Abstract

Controlled extracellular chemical and topographical cues can generate physicochemical changes that influence the proliferation and differentiation of neural cells; external electrical stimulation (ES) via conductive bioelectrodes can promote neural differentiation by increasing neurite outgrowth. Because rat pheochromocytoma (PC12) cells tend to differentiate into neuron-like cells upon treatment with nerve growth factor (NGF), we used PC12 as a model to explore the possibility of using a well-designed poly(ethylene oxide) (PEO)/poly(3,4-ethylenedioxythiophene):polystyrenesulfonate (PEDOT:PSS) blend solution to fabricate functional bioelectrodes presenting biological fouling/antifouling surfaces, thereby regulating the cell adhesion, proliferation, and differentiation properties. In this study, we found that a flat PEO/PEDOT:PSS composite film, fabricated through spin-coating, operated through a contact repulsion mechanism that limited cell attachment and proliferation; in contrast, the aligned and random PEDOT:PSS nanofibers fabricated through electrospinning promoted neuron adhesion efficiently and allowed manipulation of the cell morphology. Furthermore, we performed ES of PC12 cells to investigate the influence of the conductive random and aligned PEO/PEDOT:PSS composite nanofiber mats on the enhancement of neurite outgrowth, as well as the relative gene expression of Nestin, Tuj1, and MAP2. The PC12 cells on the aligned topography displayed predominantly bipolar neurites along the direction of the nanofibers; PC12 cells on the random nanofibers produced a greater number of neurites than did those on the aligned nanofibers; the neurite length and neuronal gene expression level were enhanced by greater than 60% relative to those of control tissue culture polystyrene plate (TCPS) substrates under ES. Therefore, combining this unique PEDOT:PSS blend solution with various fabrication processes appears to be a facile approach toward bioelectronic interface coatings displaying tunable  surface properties for manipulating the cellular behavior of neurons during ES.

2018年10月29日(週一)，1530－1720，工程一館108室

15:30-17:20 on Oct. 29 (Mon), 2018 in Room 108 of Eng. Building I

Host：Prof. Da-Jeng Yao  djyao@mx.nthu.edu.tw Ext: 42850

Contact：黃微稜 hwleng@mx.nthu.edu.tw  Ext: 33737

TA：李志恩 c27180339@gmail.com