奈米工程與微系統研究所 書報討論
Seminar of Inst. NanoEngineering and MicroSystems
2014年11月24日，下午1530－1710，工程一館１０８室
1530-1710 on Nov. 24, 2014 in Room 108 of Engineering Building I
演講者：林哲信(CH Lin)
Topic：Advanced Microscopes for Microfluidic-based Bio-analytical Applications
Abstract
High-throughput detection is always attractive for researchers in developing sensing techniques. Recently, MEMS techniques have been well-established to produce miniaturized microfluidic devices with low-cost and small size. However, large-scale conventional off-chip detection approaches may limit the throughput of the detection systems such that the benefits come with the miniaturization are hindered. A novel dark-field illumination in microscopy is used to detect the scattered light in order to monitor sample contour or detailed structure of particles. The direct light (zero-order light) is excluded from the formation of the image by illuminating the specimen with a hollow cone of light of such obliquity that all the light falls outside the acceptance angle of the objectives. In general, dark-field illumination can be created an opaque stop on light pathways through a simple refractive condenser. Instead of using the conventional laser induced fluorescence (LIF) microscope equipped with delicate spatial filters and complex control systems, this study adopts a hollow cone illumination generated using a dark-field condenser for exciting fluorescence in the microchannel and an UV-VIS-NIR spectrometer for detecting the emission signals. The proposed system is simple and economical since no sophisticated optical filter sets and laser sources are required for the detection purpose such that the system is simple and low-cost. This work reports a state-of-the-art diascopic illumination system for high-throughput multispectral analysis in microfluidic CE system and flow cytometry. A novel chromatic aberration technique for rapid detecting the Z-position of small moving objects in microfluidic channel is also presented.
Contact: Chen Yu Ju, #80627, cues91225@gmail.com