奈米工程與微系統研究所　書報討論

Seminar of Inst. NanoEngineering and MicroSystems

Prof. Da-Jeng Yao,

Department of Power Mechanical Engineering,

Institute of NanoEngineering and MicroSystems,

National Tsing Hua University

E-mail : djyao@mx.nthu.edu.tw

In Vitro Fertilization on a Chips

Abstract

The in vitro fertilization (IVF) is an important technique in biological and clinical studies since 1970. In recent years, microfluidic system has been widely used in cell detection or position a single-cell level, including the mechanical, optical, electromagnetic fields due to the advantages of biocompatibility, high-precision, low-cost, disposable, and easy to reproduce etc. In this presentation, we propose three different types of microfluidic systems, the imitation oviduct microfluidic chips, to enhance the probability of embryo fertilization for Oligozoospermia patients.

At the first of the continued microfluidic chip, the motile sperms from the oligozoospermia patients can be separated by using microfluidic chip based on its laminar flow. Then to imitate the oviduct, the oocyte can be effectively position by dielectrophoresis (DEP) technology, and it will be reacted with those motile sperms to form embryo under proper cultural environment. Finally, the reacted oocyte, embryo, was moved to incubator for further culture. The embryo can be cultured successfully after three and an half day, that is comparable with the one by using traditional IVF.

In contract with continued microfluidic system, two types of droplet-based microfluidic system would be presented. The dynamic culture is demonstrated with a system for EWOD that can manipulate a single droplet containing one mouse embryo to mimic the path from an oviduct to a uterus. A static mouse embryo culture on EWOD chips is presented for comparison with the results of dynamic culture. According to the dynamic and static results, the rate of embryo cleavage to hatching blastocyst with a dynamic culture is greater than that with a static culture. The EWOD system can enhance the culture of mouse embryos in a dynamic environment. Another droplet-based microfluidic system will also be shown, not just only for the embryo droplet formation from sperms and oocyte, but further for dynamic culture in the microchannel.

2018年12月10日(週一)，1530－1720，工程一館108室

15:30-17:20 on Dec. 10 (Mon), 2018 in Room 108 of Eng. Building I

Host：Prof. Da-Jeng Yao  djyao@mx.nthu.edu.tw Ext: 42850

Contact：黃微稜 hwleng@mx.nthu.edu.tw  Ext: 33737

TA：李志恩 c27180339@gmail.com