奈米工程與微系統研究所　書報討論

Seminar of Inst. NanoEngineering and MicroSystems

Chieh-Cheng Huang

Institute of Biomedical Engineering

National Tsing Hua University

 Assistant Professor

Injectable Cell Delivery Constructs for Myocardial Tissue Engineering

Abstract:

Cell transplantation via direct intramuscular injection is a promising therapy for patients with ischemic diseases. However, following injections, retention of transplanted cells in engrafted areas remains problematic, and can be deleterious to cell-transplantation therapy. In this presentation, a thermo-responsive hydrogel system composed of aqueous methylcellulose (MC) blended with phosphate-buffered saline is constructed to grow cell sheet fragments and cell aggregates for the treatment of ischemic diseases. The as-prepared MC hydrogel system undergoes a sol–gel reversible transition upon heating or cooling at approximately 32 °C. Via this unique property, the grown cell sheet fragments (cell aggregates) can be harvested without using proteolytic enzymes; consequently, their inherent extracellular matrices (ECMs) and integrative adhesive agents remain well preserved. In animal studies using rats and pigs with experimentally created myocardial infarction, the injected cell sheet fragments (cell aggregates) become entrapped in the interstices of muscular tissues and adhere to engraftment sites, while a minimal number of cells exist in the group receiving dissociated cells. Moreover, transplantation of cell sheet fragments (cell aggregates) significantly increases vascular density, thereby improving the function of an infarcted heart. These experimental results demonstrate that cell sheet fragments (cell aggregates) function as a cell-delivery construct by providing a favorable ECM environment to retain transplanted cells locally and consequently, improving the efficacy of therapeutic cell transplantation.

15:30-17:20 on Oct. 16(Mon), 2017 at Room 108 of Eng. Building I

Host：Prof. Chao-An Lin calin@pme.nthu.edu.tw Ext:42607

Contact：Praveen praveenbowler@gmail.com  Mobile: 0909537757

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