奈米工程與微系統研究所　書報討論

Seminar of Inst. NanoEngineering and MicroSystems

Han-Sheng Chuang

Turning Brownian Motion from Noise to Signal: An Example of Rapid Anti-Microbial Susceptibility Testing by Diffusometric Immunosensing

2016年10月24日(週一)，1530－1720，工程一館108室

1530-1720 on Oct. 24 (Mon.), 2016 in Room 108 of Eng. Building I

Abstract

Brownian motion has long been treated as random noise. However, taking advantage of the Einstein relation, the particle diameter can be related to the diffusivity change. Therefore, optical diffusometry is used to detect the existence of microorganisms in this study. By mixing target bacteria, P. aeruginosa, S. aureus, or E. coli with functionalized particles, Brownian motion is altered accordingly. The corresponding particle diffusivity can then serve as an indicator in relation to the concentration of the bacteria. The technique requires neither complicated fabrication nor sophisticated instruments. A limit of detection (LOD) as low as one bacterium per particle was achieved. The result was further used to improve the anti-microbial susceptibility testing (AST). At last, the turnaround time for each AST measurement was successfully reduced from days to hours (~2 h) based on the proposed method. The successful attempt provides insight to motoring of microorganisms in broad applications, such as water quality control and food industry. (MOST grant # 104-2221-E-006 -093 -MY3)

Contact: Wei-Chih Wang (03-5742488)