奈米工程與微系統研究所　書報討論

Seminar of Inst. NanoEngineering and MicroSystems

許佳賢 博士

Chia-Hsien Hsu

Microfluidic chip-based approach for neural stem cell manipulation

2016年10月3日(週一)，1530－1720，工程一館108室

1530-1720 on Oct. 3 (Mon.), 2016 in Room 108 of Eng. Building I

Abstract

When cultured in-vitro, the self-renewal property of neural stem cells (NSC) results into spherical clusters of neural stem cells termed neurospheres. Neurospheres consist of multipotent NSCs, and thus they provide a useful model for studying the self-renewal and proliferation of neural stem cells. In neurosphere culture, dissociation of neurospheres into single neural stem cells is an indispensable step for the subculture and analysis of neural stem cells, and is usually achieved by enzymatic dissociation or manual pipetting. In this talk, the development of a microfluidic-chip-based method for neurosphere dissociation will be presented. The applicability of this device as demonstrated by using mouse neural stem cells models will also be demonstrated. This microfluidic-chip-based cell dissociation method may provide a useful tool for stem cell research and applications.

Contact: Wei-Chih Wang (03-5742488)

Photo: